Optical Microscopy - Revision history

Cpeterson70: Add information about optical microscopy

2024-11-07T23:11:45Z

Add information about optical microscopy

← Older revision		Revision as of 19:11, 7 November 2024
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	[[File:Page-under-construction.png\|400px\|thumb\|left]]		[[File:Page-under-construction.png\|400px\|thumb\|left]]
	{\|class="infobox"		{\| class="infobox"
	!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope [https://sites.gatech.edu/mse-mill/sop/digital-microscope/ [SOP<nowiki>]</nowiki>]		!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope [https://sites.gatech.edu/mse-mill/sop/digital-microscope/ [SOP<nowiki>]</nowiki>]
	\|-		\|-
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	\|-		\|-
	\|'''Objectives''' (diagonal FOV, in mm):		\|'''Objectives''' (diagonal FOV, in mm):
			\|-
			\|    '''- PlanAPO FOV 43.75'''
			\|-
			\|        - Max. magnification: 190x
			\|-
			\|        - Max. resolution: 415 lp/mm (2.4 μm)
			\|-
			\|        - Working distance (WD): 60 mm
	\|-		\|-
	\|    '''- PlanAPO FOV 12.55'''		\|    '''- PlanAPO FOV 12.55'''
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	\|        - Max. resolution: 1073 lp/mm (0.93 μm)		\|        - Max. resolution: 1073 lp/mm (0.93 μm)
	\|-		\|-
	\|        - ~~Working distance (~~WD): 33 mm		\|        - WD: 33 mm
	\|-		\|-
	\|    '''- PlanAPO FOV 3.60'''		\|    '''- PlanAPO FOV 3.60'''
	\|-		\|-
	\|        - Max. magnification: 2350x		\|        - Max. magnification: 2350x
	\|-		\|-
	\|        - Max. resolution: 2366 lp/mm (0.42 μm)		\|        - Max. resolution: 2366 lp/mm (0.42 μm)
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	\|-		\|-
	\|'''Capabilities''': XY Image stitching, Z-stack stitching		\|'''Capabilities''': XY Image stitching, Z-stack stitching
			\|-
			\|-
			! style="font-size:135%;text-align:center;" \|AmScope Optical Stereomicroscope [https://sites.gatech.edu/mse-mill/sop/stereomicroscope/ [SOP<nowiki>]</nowiki>]
			\|-
			\|[[File:StereoOptical-052621.png\|center\|300px]]
			\|-
			\|'''Camera''': DCM300
			\|-
			\|-
			! style="font-size:135%;text-align:center;" \|Leica DMi8-M Inverted Microscope [https://sites.gatech.edu/mse-mill/sop/inverted-microscope/ [SOP<nowiki>]</nowiki>]
			\|-
			\|[[File:InvertedMicroscope-062621.png\|center\|300px]]
			\|-
			\|'''Camera''': Leica DMC2900
	\|}		\|}

	~~PAGE UNDER CONSTRUCTION~~		Optical microscopy involves the use of visible light and a series of lenses to image samples at high magnifications and resolutions. Optical microscope can vary in functionality, design, and objectives used.

			==Principle==
			Optical microscopy uses the interaction between visible light and the surface of the sample to create an image. A series of lenses can allow for the imaging of surface details normally invisible to the human eye.

			==Limitations==
			Optical microscopes are often limited by light itself. Since optical microscopes observe visual light which has wavelengths from approximately 380 to 780 nanometers, a variety of limitations arrise.

	~~{\|class~~=~~"infobox"~~		===Resolution===
	!		The resolution of optical microscopes is limited by the diffraction limit of light, preventing them from imaging beyond a certain scale. In order to image at a lower scale, [[Scanning Electron Microscope (SEM)\|scanning electron microscopy]] must be used.
	~~!style~~=~~"font-size:135%;text-align:center;" \| AmScope Optical Stereomicroscope [https://sites.gatech.edu/mse-mill/sop/stereomicroscope/ [SOP<nowiki>]</nowiki>]~~
	~~!style="font-size:135%;text-align:center;" \| Leica DMi8-M Inverted Microscope [https://sites.gatech~~.~~edu/mse-mill/sop/inverted-microscope/~~ [~~SOP<nowiki>]</nowiki>]~~
	\|-
	~~\|      \|\|~~[~~[File:StereoOptical-052621.png\|center~~\|~~300px~~]]~~\|\|[[File:InvertedMicroscope-062621~~.~~png\|center\|300px]]~~
	\|-
	~~\|'''Camera''':\|\|DCM300\|\|Leica DMC2900~~
	\|}

			===Depth of Field===
			Optical microscopes have limited depth of field due to the lenses used. This necessitates re-focusing on different heights of samples at higher magnifications. However, this limited depth of field can be used as an advantage, as described in [[#Focus/Z Stacking\|Focus/Z Stacking]]. On the Leica DVM6, different objectives with different FOVs can be used to migate this issue, however there will still be issues depth of field issues at high magnifications.

			==Advantages and Useful Properties==
			Although there are limitations while using optical microscopy, some properties of light can be taken advantage of.

			===Focus/Z Stacking===
			Using the Leica DVM6 and it's software Leica LAS X, images of a sample can be taken at different focus heights in order to create a 3D reconstruction of the sample's surface. This 3D reconstruction can be used to measure different depths of surface features or distances between different locations on the surface. Additionally, this reconstruction can be exported as a 3D mesh for use outside the software.

	[[Category:Equipment\|char]]		[[Category:Equipment\|char]]

Rzhou95 at 02:56, 6 October 2021

2021-10-06T02:56:57Z

← Older revision		Revision as of 22:56, 5 October 2021
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	[[File:Page-under-construction.png\|400px\|thumb\|left]]		[[File:Page-under-construction.png\|400px\|thumb\|left]]
	{\|class="infobox"		{\|class="infobox"
	!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope		!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope [https://sites.gatech.edu/mse-mill/sop/digital-microscope/ [SOP<nowiki>]</nowiki>]
	\|-		\|-
	\|[[File:LeicaOptical-052621.png\|center\|300px]]		\|[[File:LeicaOptical-052621.png\|center\|300px]]
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	{\|class="infobox"		{\|class="infobox"
	!		!
	!style="font-size:135%;text-align:center;" \| AmScope Optical Stereomicroscope		!style="font-size:135%;text-align:center;" \| AmScope Optical Stereomicroscope [https://sites.gatech.edu/mse-mill/sop/stereomicroscope/ [SOP<nowiki>]</nowiki>]
	!style="font-size:135%;text-align:center;" \| Leica DMi8-M Inverted Microscope		!style="font-size:135%;text-align:center;" \| Leica DMi8-M Inverted Microscope [https://sites.gatech.edu/mse-mill/sop/inverted-microscope/ [SOP<nowiki>]</nowiki>]
	\|-		\|-
	\|      \|\|[[File:StereoOptical-052621.png\|center\|300px]]\|\|[[File:InvertedMicroscope-062621.png\|center\|300px]]		\|      \|\|[[File:StereoOptical-052621.png\|center\|300px]]\|\|[[File:InvertedMicroscope-062621.png\|center\|300px]]

Rzhou95 at 19:31, 26 July 2021

2021-07-26T19:31:17Z

← Older revision		Revision as of 15:31, 26 July 2021
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			[[File:Page-under-construction.png\|400px\|thumb\|left]]
	{\|class="infobox"		{\|class="infobox"
	!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope		!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope

Rzhou95 at 18:30, 23 July 2021

2021-07-23T18:30:36Z

← Older revision		Revision as of 14:30, 23 July 2021
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	\|        - WD: 5 mm		\|        - WD: 5 mm
	\|-		\|-
	\|Lens tilt range: ±60°		\|'''Lens tilt range''': ±60°
	\|-		\|-
	\|Stage XY range: 70 mm x 50 mm		\|'''Stage XY range''': 70 mm x 50 mm
	\|-		\|-
	\|Stage rotation range: ±180°		\|'''Stage rotation range''': ±180°
	\|-		\|-
	\|Stage max load: 2 kg		\|'''Stage max load''': 2 kg
	\|-		\|-
	\|Illumination sources: 4-quadrant ring light, coaxial light		\|'''Illumination sources''': 4-quadrant ring light, coaxial light
	\|-		\|-
	\|Capabilities: XY Image stitching, Z-stack stitching		\|'''Capabilities''': XY Image stitching, Z-stack stitching
	\|}		\|}

Rzhou95 at 18:29, 23 July 2021

2021-07-23T18:29:57Z

← Older revision		Revision as of 14:29, 23 July 2021
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	{\|class="infobox"		{\|class="infobox"
	!style="font-size:135%;text-align:center;" \| Leica DVM6 Digital Microscope		!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope
	\|-		\|-
	\|[[File:LeicaOptical-052621.png\|center\|300px]]		\|[[File:LeicaOptical-052621.png\|center\|300px]]
	\|-		\|-
	\|'''~~Resolution~~''': ~~1µm~~		\|'''Objectives''' (diagonal FOV, in mm):
	\|}		\|-
	{\|~~class="infobox"~~		\|    '''- PlanAPO FOV 12.55'''
	~~!style="font~~-~~size~~:~~135%~~;~~text~~-~~align~~:~~center~~;" \| ~~AmScope Optical Stereomicroscope~~		\|-
			\|        - Max. magnification: 675x
			\|-
			\|        - Max. resolution: 1073 lp/mm (0.93 μm)
			\|-
			\|        - Working distance (WD): 33 mm
			\|-
			\|    '''- PlanAPO FOV 3.60'''
			\|-
			\|        - Max. magnification: 2350x
			\|-
			\|        - Max. resolution: 2366 lp/mm (0.42 μm)
			\|-
			\|        - WD: 5 mm
			\|-
			\|Lens tilt range: ±60°
			\|-
			\|Stage XY range: 70 mm x 50 mm
			\|-
			\|Stage rotation range: ±180°
	\|-		\|-
	\|~~[[File~~:~~StereoOptical-052621.png\|center\|300px]]~~		\|Stage max load: 2 kg
	\|-		\|-
	\|~~'''Objectives'''~~: 2x, ~~40x~~		\|Illumination sources: 4-quadrant ring light, coaxial light
	\|-		\|-
	\|~~'''Camera'''~~: ~~DCM300~~		\|Capabilities: XY Image stitching, Z-stack stitching
	\|}		\|}

			PAGE UNDER CONSTRUCTION

	{\|class="infobox"		{\|class="infobox"
			!
			!style="font-size:135%;text-align:center;" \| AmScope Optical Stereomicroscope
	!style="font-size:135%;text-align:center;" \| Leica DMi8-M Inverted Microscope		!style="font-size:135%;text-align:center;" \| Leica DMi8-M Inverted Microscope
	\|-		\|-
	\|[[File:InvertedMicroscope-062621.png\|center\|300px]]		\|      \|\|[[File:StereoOptical-052621.png\|center\|300px]]\|\|[[File:InvertedMicroscope-062621.png\|center\|300px]]
	\|-		\|-
	\|'''Camera''': Leica DMC2900		\|'''Camera''':\|\|DCM300\|\|Leica DMC2900
	\|}		\|}

	~~PAGE UNDER CONSTRUCTION~~


	[[Category:Equipment\|char]]		[[Category:Equipment\|char]]

Rzhou95 at 18:08, 23 July 2021

2021-07-23T18:08:00Z

← Older revision		Revision as of 14:08, 23 July 2021
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	\|}		\|}

	The Leica DVM 6 Microscope has some unique features. These include, being able to rotate the head of the microscope 30 degrees in either the clockwise or counter clockwise direction, generate 3D mapping of images using a stiching of images taken at multiple elevations, and being able to focus samples with rough surface topography. This microscope is very powerful and is able to resolve features on samples down to a size of 1 µm.		PAGE UNDER CONSTRUCTION

	The AmScope optical stereomicroscope is a simple optical microscope, capable of magnifying samples up to 40X. The microscope’s software allows for digital images and videos to be taken and stored on the computer.

	The Leica DMi8-M Inverted Microscope flips the location of the light source and objective from traditional microscopy. This guarantees a level viewing plane, and is ideal for polished metal samples and live cells. Our setup uses a Leica DMC2900 camera for imaging.


	[[Category:Equipment\|char]]		[[Category:Equipment\|char]]

Rzhou95 at 00:06, 18 July 2021

2021-07-18T00:06:11Z

← Older revision		Revision as of 20:06, 17 July 2021
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	\|-		\|-
	\|'''Objectives''': 2x, 40x		\|'''Objectives''': 2x, 40x
			\|-
			\|'''Camera''': DCM300
	\|}		\|}
	{\|class="infobox"		{\|class="infobox"

Rzhou95: Rzhou95 moved page Light Microscope to Optical Microscopy

2021-06-29T14:41:53Z

Rzhou95 moved page Light Microscope to Optical Microscopy

← Older revision		Revision as of 10:41, 29 June 2021
(No difference)

Rzhou95 at 14:38, 29 June 2021

2021-06-29T14:38:47Z

← Older revision		Revision as of 10:38, 29 June 2021
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	\|}		\|}
	{\|class="infobox"		{\|class="infobox"
	!style="font-size:135%;text-align:center;" \| Optical Stereomicroscope		!style="font-size:135%;text-align:center;" \| AmScope Optical Stereomicroscope
	\|-		\|-
	\|[[File:StereoOptical-052621.png\|center\|300px]]		\|[[File:StereoOptical-052621.png\|center\|300px]]

Rzhou95 at 14:38, 29 June 2021

2021-06-29T14:38:26Z

@@ Line 5: / Line 5: @@
 |-
 |'''Resolution''': 1µm
 |}
-The Leica DVM 6 Microscope has some unique features. These include, being able to rotate the head of the microscope 30 degrees in either the clockwise or counter clockwise direction, generate 3D mapping of images using a stiching of images taken at multiple elevations, and being able to focus samples with rough surface topography.
+The Leica DVM 6 Microscope has some unique features. These include, being able to rotate the head of the microscope 30 degrees in either the clockwise or counter clockwise direction, generate 3D mapping of images using a stiching of images taken at multiple elevations, and being able to focus samples with rough surface topography. This microscope is very powerful and is able to resolve features on samples down to a size of 1 µm.
 [[Category:Equipment|char]]

← Older revision		Revision as of 22:56, 5 October 2021
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	[[File:Page-under-construction.png\|400px\|thumb\|left]]		[[File:Page-under-construction.png\|400px\|thumb\|left]]
	{\|class="infobox"		{\|class="infobox"
	!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope		!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope [https://sites.gatech.edu/mse-mill/sop/digital-microscope/ [SOP<nowiki>]</nowiki>]
	\|-		\|-
	\|[[File:LeicaOptical-052621.png\|center\|300px]]		\|[[File:LeicaOptical-052621.png\|center\|300px]]
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	{\|class="infobox"		{\|class="infobox"
	!		!
	!style="font-size:135%;text-align:center;" \| AmScope Optical Stereomicroscope		!style="font-size:135%;text-align:center;" \| AmScope Optical Stereomicroscope [https://sites.gatech.edu/mse-mill/sop/stereomicroscope/ [SOP<nowiki>]</nowiki>]
	!style="font-size:135%;text-align:center;" \| Leica DMi8-M Inverted Microscope		!style="font-size:135%;text-align:center;" \| Leica DMi8-M Inverted Microscope [https://sites.gatech.edu/mse-mill/sop/inverted-microscope/ [SOP<nowiki>]</nowiki>]
	\|-		\|-
	\|      \|\|[[File:StereoOptical-052621.png\|center\|300px]]\|\|[[File:InvertedMicroscope-062621.png\|center\|300px]]		\|      \|\|[[File:StereoOptical-052621.png\|center\|300px]]\|\|[[File:InvertedMicroscope-062621.png\|center\|300px]]

← Older revision		Revision as of 15:31, 26 July 2021
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			[[File:Page-under-construction.png\|400px\|thumb\|left]]
	{\|class="infobox"		{\|class="infobox"
	!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope		!style="font-size:135%;text-align:center;"\| Leica DVM6 Digital Microscope